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High Pressure Liquid Chromatography (HPLC)

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By: DAVID SAVASTANO

Contributing Editor, Coatings World and Ink World, Coatings World and Ink World Magazine

A separation technique based on a solid stationary phase and a liquid mobile phase. Separations (into distinct bands) are achieved by partition, adsorption, or ion-exchange processes, depending upon the type of stationary phase used. Each band is then profiled as the solvent flows through a UV detector, or by fluorescence, or refractive index detectors. Sometimes called high-performance liquid chromatography.

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